KMID : 1025520170590010016
|
|
Journal of Animal Science and Technology 2017 Volume.59 No. 1 p.16 ~ p.16
|
|
Role of ghrelin in the pancreatic exocrine secretion via mitogen-activated protein kinase signaling in rats
|
|
Lee Kyung-Hoon
Lee Jae-Sung Wang Tao Oh Jin-Ju Roh Sang-Gun Lee Hong-Gu
|
|
Abstract
|
|
|
Background: This study was performed to investigate the impact of exogenous ghrelin on the pancreatic ¥á-amylase outputs and responses of pancreatic proteins to ghrelin that may relate to pancreatic exocrine.
Methods: Sprague-Dawley male rats (9 weeks old, 300 ¡¾ 10 g) were injected with ghrelin via intraperitoneal (i.p.) infusion at dosage of 0, 0.1, 1.0 and 10.0 ¥ìg/kg body weight (BW), respectively. The plasma ghrelin and cholecystokinin (CCK) level were determined using enzyme immunoassay kit; the mRNA expression of ghrelin receptor (GHSR-1¥á) and growth hormone (GH) receptor were assessed by reverse transcription PCR; the expressions of pancreatic ¥á-amylase activity, extracellular-signal-regulated kinases (ERK), phosphorylated extracellular-signal-regulated kinases (pERK) and c-Jun N-terminal kinase (JNK) were evaluated by western blotting; moreover the responses of pancreatic proteins to ghrelin were analyzed using the two-dimensional gel electrophoresis system.
Results: The exogenous ghrelin (1.0 and 10.0 ¥ìg/kg BW) elevated the level of plasma ghrelin (p < 0.05), and suppressed the expression of pancreatic ¥á-amylase at a dose of 10.0 ¥ìg/kg BW (p < 0.05). No difference in the level of plasma CCK was observed, even though rats were exposed to any dose of exogenous ghrelin. In addition, a combination of western blot and proteomic analysis revealed exogenous ghrelin (10.0 ¥ìg/kg BW) induced increasing the JNK and ERK expressions (p < 0.05) and four proteins such as Destrin, Anionic trypsin-1, Trypsinogen, and especially eukaryotic translation initiation factor 3 in rat pancreas.
Conclusions: Taken together, exogenous ghrelin by i.p. infusion plays a role in the pancreatic exocrine secretion via mitogen-activated protein kinase signaling pathway.
|
|
KEYWORD
|
|
¥á-Amylase activity, Cholecystokinin, Ghrelin, Pancreatic exocrine, Sprague-Dawley rats
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|